Figure/Graphic 1a-c: Molecular markers in glioma
Molecular markers in glioma. A, 1p/19q co-deletion. Microsatellite-PCR-based analysis of allelic losses of 1p and 19q in an astrocytoma (WHO grade II) (A II) and an anaplastic oligodendroglioma (WHO grade III) (AO III). Losses of both markers are indicated by arrowheads in AO III, but not A II. B, MGMT promoter methylation. Methylation-specific PCR for unmethylated (U) and methylated (M) promoter sequences in 5 glioblastoma samples, including the glioblastoma cell line A172 as a positive control for methylated and peripheral blood cells as a control for unmethylated promoter sequences, as well as water as a negative control (empty). C, IDH mutation. Grade- II oligoastrocytoma, upper panels: HE staining, lower panels: IDH immunostaining, left panels: tumour centre, right panels: infiltration zone (Courtesy: Jörg Felsberg, Düsseldorf, Germany). Reprinted from [2].