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Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16

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Zum ersten Bild Abb. 2: Soamtic cell nuclear transfer Abb. 3: Somatic cell nuclear transfer Abb. 4: Interspecies-SCNT Aktuelles Bild - Abb. 5a-b: DNA sequence analysis Abb. 6a-c: DNA sequence analysis Abb. 7: Splitting - Early Embryo Abb. 8: Mouse donor embryo Zum letzten Bild
Abbildung 5a-b: DNA sequence analysis
Human genomic DNA sequence analysis of interspecies-cloned embryo and human adult fibroblast donor cells used for SCNT. Identical DNA sequence profiles concerning peak positions are detectable for interspecies embryo (A) and human donor cells (B). Some variations in peak levels result from different sample analysis. Three chromosomal microsatellite probes were used for PCR amplification. FGA (chromosome 4q28), D21S11 (chromosome 21), D13S317 (chromosome 13q22–31). A standard marker (ABI Applied Biosystem ROX Reference Dye) served as internal control. For further details see [78].
 
DNA sequence analysis
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Abbildung 5a-b: DNA sequence analysis
Human genomic DNA sequence analysis of interspecies-cloned embryo and human adult fibroblast donor cells used for SCNT. Identical DNA sequence profiles concerning peak positions are detectable for interspecies embryo (A) and human donor cells (B). Some variations in peak levels result from different sample analysis. Three chromosomal microsatellite probes were used for PCR amplification. FGA (chromosome 4q28), D21S11 (chromosome 21), D13S317 (chromosome 13q22–31). A standard marker (ABI Applied Biosystem ROX Reference Dye) served as internal control. For further details see [78].
 
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