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1. Koyama K; Hasegawa A
Premature Ovarian Failure Syndrome May Be Induced by Autoimmune Reactions to Zona Pellucida Proteins
POF - oocytes
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2006; 3 (2): 94-97
Immunofluorescent staining of human oocytes with sera from POF and the other infertile patients. Isolated human oocytes prepared as described in Fig. 2 were treated with patients’ diluted sera for one hour at room temperature. After washing with PBS, the treated oocytes were further incubated in FITC-conjugated anti-human IgG for 50 minutes. Observations were carried out under a fluorescent microscope. Each number corresponds to the serum samples in Fig. 2. The immunofluorescent intensities did not correlate to the values in the dot immunoassay. (1)–(5) are oocytes stained with POF patients’ sera, (6) and (7) are oocytes stained with other infertile patients’ sera. (8) is an oocyte stained with a healthy male’s serum showing a negative reaction in dot immunoassay.

Keywords: oocyte,  Oozyte,  POF,  premature ovarian failure
2. Koyama K; Hasegawa A
Premature Ovarian Failure Syndrome May Be Induced by Autoimmune Reactions to Zona Pellucida Proteins
Anti-Zona pellucida antibodies
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2006; 3 (2): 94-97
Detection of anti-ZP antibodies in POF patients by dot immunoassay. The human zona pellucida was isolated from unfertilised oocytes in a clinical program of IVF-ET under informed consent. Solubilised protein (0.01 µg) was dotted onto a nitrocellulose membrane. The membrane was incubated with the patients’ diluted sera and subsequently treated with an anti-human IgG antibody conjugated with horseradish peroxidase. Color development was performed by chloronaphthol and H2O2. Densitometric values showing more than twice over the SD values of the control group (healthy males) were assessed as positive. 50 % (5/10) of POF patients showed positive reaction for anti-zona antibodies, while 11.8 % (2/17) were positive in the other infertile women.

Keywords: antibody,  Antikörper,  Diagramm,  flowchart,  POF,  premature ovarian failure,  Zona pellucida
 

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