1.
Hengstschläger M
Stem Cells in Amniotic Fluid - What are the Next Steps to Do?
Oct-4 - Nucleus
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2005; 2 (4): 233-238
Detection of Oct-4 in the nucleus of a human amniotic fluid cell. A phase contrast picture of an amniotic fluid cell population grown under culture conditions used for routine prenatal diagnosis of genetic mutations is presented. For immunocytochemical analysis of cellular
Oct-4 expression, cells were fixed and incubated with anti-Oct-4 antibody. Thereafter, cells were washed, incubated with a biotinylated
secondary antibody, washed again, and incubated with
ExtrAvidin-
Cy3 conjugate. One amniotic fluid cell with the red Oct-4-specific signal in the nucleus is shown.
Keywords:
Endokrinologie,
nucleus,
Nukleus,
oct-4,
phase contrast,
Phasenkontrast,
Pluripotenzgen Oct-4
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