3.
Koyama K;
Hasegawa A
Premature Ovarian Failure Syndrome May Be Induced by Autoimmune Reactions to Zona Pellucida Proteins
POF - oocytes
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2006; 3 (2): 94-97
Immunofluorescent staining of human oocytes with sera from POF and the other infertile patients. Isolated human oocytes prepared
as described in Fig. 2 were treated with patients’ diluted sera for one hour at room temperature. After washing with PBS, the treated oocytes
were further incubated in FITC-conjugated anti-human IgG for 50 minutes. Observations were carried out under a fluorescent microscope.
Each number corresponds to the serum samples in Fig. 2. The immunofluorescent intensities did not correlate to the values in the dot immunoassay.
(1)–(5) are oocytes stained with POF patients’ sera, (6) and (7) are oocytes stained with other infertile patients’ sera. (8) is an oocyte
stained with a healthy male’s serum showing a negative reaction in dot immunoassay.
Keywords:
oocyte,
Oozyte,
POF,
premature ovarian failure