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Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16

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Zum ersten Bild Abb. 2: Soamtic cell nuclear transfer Abb. 3: Somatic cell nuclear transfer Abb. 4: Interspecies-SCNT Abb. 5a-b: DNA sequence analysis Aktuelles Bild - Abb. 6a-c: DNA sequence analysis Abb. 7: Splitting - Early Embryo Abb. 8: Mouse donor embryo Abb. 9: Blastocysts - Mouse embryo
Abbildung 6a-c: DNA sequence analysis
DNA sequence analysis of human and bovine mitochondrial (mt) DNA. From human donor cells and interspeciescloned embryo (blastocyst). For detection of human and bovine mtDNA, species-specific primers were used that amplify parts of the mtD-loop region, respectively. The amplicons were sequenced using internal primers and products separated on an ABI3100. (A) Human mtD-loop region (position 16191- 16239; GeneBank acc. no. AY275537) that was amplified from the human donor cells. (B) Human mtD-loop region obtained from interspecies-cloned embryo. Both mtDNA sequences show identical profiles. (C) Bovine mtDNA sequence from interspeciescloned embryo obtained by using bovine mtDNA primers for PCR amplification of mtD-loop region (position 306 to 359; GeneBank acc. no. AF499248). For further details see [78].
 
DNA sequence analysis
Vorheriges Bild Nächstes Bild   


Abbildung 6a-c: DNA sequence analysis
DNA sequence analysis of human and bovine mitochondrial (mt) DNA. From human donor cells and interspeciescloned embryo (blastocyst). For detection of human and bovine mtDNA, species-specific primers were used that amplify parts of the mtD-loop region, respectively. The amplicons were sequenced using internal primers and products separated on an ABI3100. (A) Human mtD-loop region (position 16191- 16239; GeneBank acc. no. AY275537) that was amplified from the human donor cells. (B) Human mtD-loop region obtained from interspecies-cloned embryo. Both mtDNA sequences show identical profiles. (C) Bovine mtDNA sequence from interspeciescloned embryo obtained by using bovine mtDNA primers for PCR amplification of mtD-loop region (position 306 to 359; GeneBank acc. no. AF499248). For further details see [78].
 
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