Exposure Steps CPs
Abbildung 1: Exposure to the CPs for vitrification and to sucrose solutions for warming of MII oocytes and zygotes. EG: ethylene glycol; DMSO: Dimethylsulfoxide


Keywords: CPs,  cryoprotectant solutions,  MII Oocyte,  Zygote

Vitrification in open and closed devices
Abbildung 2: Vitrification in open and closed devices following contact with sterile or non-sterile liquid nitrogen (LN2).


Keywords: closed system,  LN2,  open system,  vitrification

Device for aseptic vitrification
Abbildung 3a: Device for aseptic vitrification. The Vitrisafe carrier device consists of the carrier (Vitrisafe plug) (A) and a high security straw 0.3 m (B). G: Gutter to place embryos; B: Aseptic Vitrisafe CBS Straw; C: Aseptic sealed Vitrisafe device; D: Welding points.


Keywords: vitrification,  Vitrisafe

Vitrification procedure
Abbildung 3b: Vitrification procedure with the Vitrisafe plug (1). The biological material is placed on the gutter of the Vitrisafe plug (2). The Vitrisafe plug is inserted into the high security straw (3). Thermo-sealing of the outer straw (4). Hermetically closed vitrification straw (5). Plunged into liquid nitrogen (LN2)


Keywords: vitrification,  Vitrisafe

Warming process
Abbildung 4: Low warming rates and intracellular formation of ice crystals. Warming process from (A) a vitrified state; (B) devitrification; (C) freezing; (D) re-crystallization; (E) thawing.


Keywords: vitrification,  warming process