Illmensee K Mammalian Cloning and its Discussion on Applications in Medicine Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16 Volltext (PDF) Summary Übersicht
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Abbildung 6a-c: DNA sequence analysis DNA sequence analysis of human and bovine mitochondrial (mt) DNA. From human donor cells and interspeciescloned embryo (blastocyst). For detection of human and bovine mtDNA, species-specific primers were used that amplify parts of the mtD-loop region, respectively. The amplicons were sequenced using internal primers and products separated on an ABI3100. (A) Human mtD-loop region (position 16191- 16239; GeneBank acc. no. AY275537) that was amplified from the human donor cells. (B) Human mtD-loop region obtained from interspecies-cloned embryo. Both mtDNA sequences show identical profiles. (C) Bovine mtDNA sequence from interspeciescloned embryo obtained by using bovine mtDNA primers for PCR amplification of mtD-loop region (position 306 to 359; GeneBank acc. no. AF499248). For further details see [78]. |
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Abbildung 6a-c: DNA sequence analysis
DNA sequence analysis of human and bovine mitochondrial (mt) DNA. From human donor cells and interspeciescloned embryo (blastocyst). For detection of human and bovine mtDNA, species-specific primers were used that amplify parts of the mtD-loop region, respectively. The amplicons were sequenced using internal primers and products separated on an ABI3100. (A) Human mtD-loop region (position 16191- 16239; GeneBank acc. no. AY275537) that was amplified from the human donor cells. (B) Human mtD-loop region obtained from interspecies-cloned embryo. Both mtDNA sequences show identical profiles. (C) Bovine mtDNA sequence from interspeciescloned embryo obtained by using bovine mtDNA primers for PCR amplification of mtD-loop region (position 306 to 359; GeneBank acc. no. AF499248). For further details see [78]. |