Abbildung 2: Anti-Zona pellucida antibodies
Detection of anti-ZP antibodies in POF patients by dot immunoassay. The human zona pellucida was isolated from unfertilised oocytes in a clinical program of IVF-ET under informed consent. Solubilised protein (0.01 µg) was dotted onto a nitrocellulose membrane. The membrane was incubated with the patients’ diluted sera and subsequently treated with an anti-human IgG antibody conjugated with horseradish peroxidase. Color development was performed by chloronaphthol and H2O2. Densitometric values showing more than twice over the SD values of the control group (healthy males) were assessed as positive. 50 % (5/10) of POF patients showed positive reaction for anti-zona antibodies, while 11.8 % (2/17) were positive in the other infertile women.