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Koyama K, Hasegawa A Premature Ovarian Failure Syndrome May Be Induced by Autoimmune Reactions to Zona Pellucida Proteins Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2006; 3 (2): 94-97 Volltext (PDF) Summary Übersicht
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Abbildung 4: Antibodies - ELISA Increase of antibody titers in ELISA against the synthetic peptide used for immunisation. An 18-mer synthetic peptide of dog ZP2 (88–106 amino acids) was prepared and conjugated with a carrier protein, diphtheria toxoid for immunisation. The immunogen (500 µg) was injected with complete Freund’s adjuvant into three 3-month-old dogs at one-month intervals. Antibody titers were determined by ELISA against the cognate peptide antigen. |
Abbildung 4: Antibodies - ELISA
Increase of antibody titers in ELISA against the synthetic peptide used for immunisation. An 18-mer synthetic peptide of dog ZP2 (88–106 amino acids) was prepared and conjugated with a carrier protein, diphtheria toxoid for immunisation. The immunogen (500 µg) was injected with complete Freund’s adjuvant into three 3-month-old dogs at one-month intervals. Antibody titers were determined by ELISA against the cognate peptide antigen. |