Krause und Pachernegg
Verlag für Medizin und Wirtschaft
Artikel   Bilder   Volltext

Mobile Version
A-  |   A  |   A+
Werbung
 
Summary
Holzwart E et al.  
Effect of Betablockers on the Regulation of PDK (Pyruvate Dehydrogenase Kinase) Gene Expression in Both Normoxic and Hypoxic Myocardium

Journal of Clinical and Basic Cardiology 2010; 13 (1-4): 12-18

PDF    Summary    Figures   

Fig. 1: Gene expression Fig. 2: Gene expression Fig. 3: PDK Fig. 4: PCR - PDK



Keywords: betablockersheartmetabolismPDK

Pyruvate dehydrogense kinase isoforms inhibit pyruvate dehydrogenase, which constitutes an important step in glucose metabolism. It is involved in various phenomena of aging and its expression changes with age – a mechanism that is so far not well-understood. Cardiac metabolism of glucose is very tightly controlled in order to maintain the variable energy demand that is required by cardiac tissue. Energy metabolism of the cardiac myocyte can be regulated within seconds up to a few minutes or chronically regulated within the time frame of hours to days. Glucose metabolism is activated in early myocardial ischemia and in response to an increased need of high-energy phosphate in the healthy heart during extreme physical activity. In myocardial ischemia, inhibition of PDK expression would be beneficial in order to shift the myocardial metabolism from the adult towards the fetal phenotype, thus metabolising more glucose than fat to preserve myocardial integrity. Myocardial tissue probes derive from the right auricle of patients undergoing cardiac surgery. A small part of the right auricle is removed when the heart is put on extra-corporal circulation. This sample is then placed in cooled Tyrode solution and hypoxia is brought about by switching 100 % oxygen to 100 % nitrogen (hypoxia) in one of the 2 chambers. By doing so, we are able to compare ischemic and non-ischemic tissues of the same patient. Snap-frozen samples are stored at –170 °C until RNA isolation. Quality of isolated RNA is analysed by means of the Agilent’s Bioanalyzer 2100 system. Arrays are scanned with the AB1700 Chemiluminescence Array Reader and images as well as data are processed using the PANTHER software. In our microarray experiments, we find that, in particular, PDK isoform 4 is significantly less expressed under nebivolol both during O2 perfusion and simulated ischemia, an effect practically negligible under atenolol. Here, nebivolol also exhibits a unique cardioprotective property different from standard betablockers. We find that without the influence of betablockers there is no significant regulation of PDK expression during myocardial ischemia. There is merely a trend towards a decrease in PDK gene expression. There is, however a significant difference between the expression of PDK during myocardial ischemia in the presence of atenolol (3.62 ± 0.18) and nebivolol (1.97 ± 0.06; + SEM; P ≤0.05): PDK expression is decreased during normoxia (trend) and ischemia (significant) in the presence of nebivolol. Here, confirmed by real-time PCR, the finding that PDK gene expression is down-regulated by nebivolol compared to atenolol in normoxia (trend, not statistically significant) and simulated ischemia/hypoxia (statistically significant) may argue for a higher protective, anti-ischemic but also anti-anginal metabolic potential of nebivolol compared to standard betablockers like atenolol. Especially patients with angina may profit from this particular property of nebivolol over atenolol.
 
copyright © 2003–2017 Krause & Pachernegg GmbH | Sitemap | Impressum
 
Werbung