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1. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Blastocysts - Mouse embryo
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Hatching blastocysts derived from mouse embryo splitting at the 2-cell (A and B) and 4-cell
(C and D) stage. Donor blastocysts on the left and recipient blastocysts on the right. Keywords: Andrologie,
blastozyst,
Blastozyste,
Embryo,
Embryo,
Embryologie,
Genetik
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2. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Mouse donor embryo
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Blastomere biopsy from 2-cell and 4-cell mouse donor embryos (A and C) and blastomere
reinjection into empty zona pellucida recipients (B and D). Keywords: Andrologie,
balstomere,
Biopsie,
biopsy,
Blastom,
Embryo,
Embryologie,
Genetik
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3. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Splitting - Early Embryo
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Splitting of early embryos (twinning) for various applications in reproductive and therapeutic
medicine. Keywords: Andrologie,
Embryo,
embryo splitting,
Embryologie,
Genetik,
Schema,
scheme
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4. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
DNA sequence analysis
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
DNA sequence analysis of human and bovine mitochondrial (mt) DNA. From human donor cells and interspeciescloned embryo (blastocyst). For detection of human and bovine mtDNA, species-specific primers were used that amplify parts of the mtD-loop region, respectively. The amplicons were sequenced using internal primers and products separated on an ABI3100.
(A) Human mtD-loop region (position 16191-
16239; GeneBank acc. no. AY275537) that was
amplified from the human donor cells. (B) Human
mtD-loop region obtained from interspecies-cloned
embryo. Both mtDNA sequences show identical
profiles. (C) Bovine mtDNA sequence from interspeciescloned embryo obtained by using bovine mtDNA primers for PCR amplification
of mtD-loop region (position 306 to 359;
GeneBank acc. no. AF499248). For further
details see [78]. Keywords: Andrologie,
bovine embryo,
DNA,
DNA-sequence analysis,
Embryo,
Embryologie,
Genetik,
human embryo
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5. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
DNA sequence analysis
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Human genomic DNA sequence analysis of interspecies-cloned embryo and human adult fibroblast donor cells used for SCNT. Identical
DNA sequence profiles concerning peak positions are detectable for interspecies embryo (A) and human donor cells (B). Some variations in peak
levels result from different sample analysis. Three chromosomal microsatellite probes were used for PCR amplification. FGA (chromosome 4q28),
D21S11 (chromosome 21), D13S317 (chromosome 13q22–31). A standard marker (ABI Applied Biosystem ROX Reference Dye) served as internal
control. For further details see [78]. Keywords: Andrologie,
cell donor,
DNA,
DNA-sequence analysis,
Embryo,
Embryologie,
Genetik,
interspecies-cloned embryo,
Klon
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6. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Interspecies-SCNT
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Interspecies-SCNT preimplantation embryos derived from human granulosa cells fused
with enucleated bovine oocytes. Cleavage embryos (A) and blastocysts (C) derived from SCNT. Parthenogenetically developed cleavage embryos (B) and hatching blastocysts (D) as controls. Keywords: Andrologie,
Embryologie,
Genetik,
SCNT,
somatic cell nuclear transfer,
somatischer Zellkerntransfer
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7. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Somatic cell nuclear transfer
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Interspecies SCNT. (A) Enucleation of bovine oocyte at metaphase II; (B) Selection of
adult human donor cell; (C) Injection of adult human donor cell into the perivitelline space. (D)
Human donor cell attached to the enucleated bovine oocyte. Keywords: Andrologie,
Embryologie,
Genetik,
SCNT,
soamtischer Zellkern-Transfer,
somatic cell nuclear transfer
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8. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Soamtic cell nuclear transfer
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Somatic cell nuclear transfer (SCNT) for establishing embryonic stem (ES) cells in therapeutic medicine. Keywords: Andrologie,
Embryologie,
Genetik,
Schema,
scheme,
SCNT,
somatic cell nuclear transfer,
somatischer Zellkerntransfer
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9. Illmensee K
Mammalian Cloning and its Discussion on Applications in Medicine
Mammalian embryos
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2007; 4 (1): 6-16
Biotechnology on mammalian embryos at various stages of preimplantation development with
multiple applications in reproductive and therapeutic medicine. Keywords: Andrologie,
Embryo,
Embryologie,
Genetik,
mammalian embryo,
Schema,
scheme
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