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Medizinische Publikationen (4)  ::   Abbildungen und Graphiken  ::   Volltext (0)

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1. Montag M; Toth B; Strowitzki T
New Laboratory Techniques in Reproductive Medicine
Series of time-lapse images
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2013; 10 (Sonderheft 1): 33-37
Series of time-lapse images taken from one embryo starting at the 2PN stage (upper left) up to the expanded blastocyst stage (lower right). Images were taken at 19.1, 33.1, 47.1, 70.3, 89.3 and 114.1 hours after ICSI and following transfer this embryo implanted and gave a pregnancy. Analysis of cleavage times and intervals between cleavages allows for subtle differentiation between individual embryos and helps choosing the one with the most synchronous and timed development.

Keywords: 2PN stage,  blastocyst stage,  time-lapse
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Series of time-lapse images

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2. Montag M; Toth B; Strowitzki T
New Laboratory Techniques in Reproductive Medicine
Result of an array-CGH analysis
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2013; 10 (Sonderheft 1): 33-37
This example shows the result of an array-CGH analysis of the 1st polar body from an oocyte taken from a patient with a maternal reciprocal translocation 46,XX,t(5;8)(q22;p11.2). The possibility to detect numerical (loss of a chromatid 15 and 16) and structural chromosomal aberrations (gain of a chromatid 8p and loss of a chromatid 5q) by array-CGH is obvious. Reprinted in part with permission from [Montag et al., J Reproduktionsmed Endokrinol 2010; 6: 498–502].

Keywords: array-CGH
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Result of an array-CGH analysis

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3. Montag M; Toth B; Strowitzki T
Sperm Selection in ART
Sperm selection
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2012; 9 (6): 485-489
Sperm selection by hyaluronic acid binding. The figure shows a dish where the lower right part is coated with hyaluronic acid. Sperm with hyaluronic acid binding sites attach to the coated part and are expected to be mature and suitable for ICSI. Spermatozoa at the upper left corner have not bound and are still motile. These sperm are immature and should not be used for ICSI.

Keywords: hyaluronic acid binding,  ICSI,  sperm
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Sperm selection

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4. Montag M; Toth B; Strowitzki T
Sperm Selection in ART
Sperm
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2012; 9 (6): 485-489
Comparison of digital enhanced high magnification image versus conventional Hoffman modulation contrast. This figure shows a picture of two sperm taken with a standard Hofmann 40× objective (A) and a 63× objective with a high numerical aperture and using Cytoscreen (B) a digital image enhancement software (Octax, Bruckberg, Germany). The presence of a small vacuole in the head of the right sperm as visualized by digital enhanced high magnification can be seen in the standard Hofmann contrast picture as a irregularity in the sperm head. Reprinted from [23].

Keywords: sperm,  vacuole
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Sperm

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5. Montag M; Toth B; Strowitzki T
Sperm Selection in ART
Sperm viability
Journal für Reproduktionsmedizin und Endokrinologie - Journal of Reproductive Medicine and Endocrinology 2012; 9 (6): 485-489
Sperm viability as proven by laser. A viable sperm is shown after the application of a single laser shot. The laser shot caused a curling of the end of the sperm tail which is indicative for sperm viability. Nonviable sperm show no reaction of the sperm tail. Despite the curling of the sperm tail these sperm can be used for ICSI without any further treatment as the laser causes also a permeabilization of the sperm plasma membrane [15].

Keywords: single laser shot,  sperm
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Sperm viability

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